After Sheen's bombshell announcement on Tuesday, Mueller's rep says she does not have the infection.
Charlie Sheen's ex-wives are speaking out confirming they do not have HIV following his bombshell revelation on the Today show on Tuesday.
Sheen split from Brooke Mueller, the mother of his twin boys, in 2011 - the same year he says he was diagnosed as HIV positive.
But her spokesperson said on Tuesday: "Brooke can confirm that she and the boys are not HIV positive."
Read: Charlie Sheen Reveals He is HIV Positive: 'It's a Hard 3 Letters to Absorb'
Denise Richards, with whom Sheen has two daughters, has known he has been HIV positive for several years.
According to a source close to the former Bond girl: "He was infected after they divorced, and they haven't been intimate since. Neither she nor their daughters are HIV-positive."
Sheen also has a 30-year-old daughter, Cassandra, from his first marriage.
On the Today show, he talked about her, saying: “It hit her hard. I said, 'I’m sorry I didn't tell you sooner, but it didn't seem like you could do anything and I didn't want to burden you with all the stress.' But she was a rock star about this."
Sheen's friends are expressing support.
His former Spin City co-star Heather Locklear posted a message on Instagram:
But many are pointing out that Sheen only has himself to blame after a lifetime of debauchery and out-of-control behavior.
His antics cost him his role on Two and a Half Men and sparked a very public meltdown.
During that time he was spotted swinging a machete around on a rooftop in Hollywood. His L.A. mansion, where he lived with a revolving cast of so-called "goddesses," was party central.
INSIDE EDITION's Steven Fabian spoke to Dr. Oz, who will air an interview he carried out with the actor from 2013 on Wednesday.
Dr. Oz told Fabian: "Drinking alochol and doing drugs were a much bigger risks for his life than his HIV status."
Sheen says he continued to have sex, even after his diagnosis, but says he took precautions.
Read: HIV-Positive Man Accused of Raping 6-Year-Old Boy, While Cops Say Another Man Videotaped It
In the Dr. Oz episode, Sheen said he used protection when he had sex with different women.
Sheen told Today show's Matt Lauer that it was "impossible" he could have transmitted the HIV virus to anyone.
Fabian asked Dr. Oz if that could be the case. "The risks of him transmitting the virus, right now, are about the same as being struck by lightning," Dr. Oz said.
Sheen's own doctor seems upbeat about his future saying: “He is healthy. He does not have AIDS."
But is the outlook really that upbeat for Charlie Sheen?
According to Dr. Oz: "I am here to say very loudly that you might think HIV is a death sentence, it is not. HIV is a lot of chronic illnesses. You can live a very long life if you take care of yourself but each day of your life will be normal as well."
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