Jared Knabenbauer, known professionally as ProJared, has come under scrutiny lately.
He later posted a statement about divorcing his wife, cosplayer and fashion designer Heidi O'Ferrall.
In the statement on Twitter, Knabenbauer said in part that "during this time you may see a lot of rumors, speculation, and gossip going around."
"I ask that you make your own observations and come to your own conclusions," he added.
But his estranged wife fired back.
After claiming that Knabenbauer blocked her from seeing his tweets, she publicly accused him of having an affair with another YouTube star.
In a thread of tweets, O'Ferrall claimed to have photographic proof of the affair and said the past year of her marriage has been isolating and full of lies on behalf of her husband.
Knabenbauer has not responded to the accusations, but many of his fans seem to be jumping ship, with thousands lost already.
Knabenbauer has not responded to InsideEdition.com's request for comment.